phosphorylation assay kit  (Sangon Biotech)

Journal: Poultry Science

Article Title: Heterologous expression, immunogenic evaluation, and subunit vaccine potential of the σC protein from the Xinjiang avian reovirus (ARV) strain xj-1.1

doi: 10.1016/j.psj.2026.106779

Figure Lengend Snippet: Standard curve for phosphorylation level determination of recombinant σC proteins.

Article Snippet: Phosphorylation levels were quantified using the formula provided by the Phosphorylation Assay Kit (purchased from Sangon Biotech (Shanghai) Co., Ltd., catalog number: C500061): N (Protein phosphorylation level%) = (X × 2.24% × A)/(B × 31) Where: N: Molar percentage of phosphorus per mole of the target phosphorylated protein; A: Molecular weight of the target protein (g/mol); B: Total protein concentration of the sample (μg/mL); X: Phosphorylated protein concentration of the sample (μg/mL); Note: The phosphorylation level of the phosphorylated standard protein used in this assay was 2.24%.

Techniques: Phospho-proteomics, Recombinant

Journal: Molecules

Article Title: Discovery of a Novel Coumarin/Thiazole Chalcone Hybrid as a Potent Dual Inhibitor of Tubulin and Carbonic Anhydrases IX & XII with Promising Anti-Proliferative Activity

doi: 10.3390/molecules31060917

Figure Lengend Snippet: Effect of compound 6 on VEGF-stimulated VEGFR-2 (Tyr1175) phosphorylation in HUVEC cells.

Article Snippet: Phosphorylated VEGFR-2 (Tyr1175) levels were quantified using the PathScan ® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit (#7335, Cell Signaling Technology, Danvers, MA, USA) following the rapid protocol.

Techniques: Phospho-proteomics

Journal: Journal of Nanobiotechnology

Article Title: ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata

doi: 10.1186/s12951-026-04214-7

Figure Lengend Snippet: ( a ) Isolation and characterization of UMSC-EVs. ( b ) Isolation and characterization of EMSC-EVs. ( c ) Characterization of UMSC-EVs and EMSC-EVs by WB. ( d ) AA mouse model. ( e ) H&E staining of skin from AA mice. ( f ) H&E staining of skin from healthy mice. ( g ) Hair growth in each group on day 15. ( h ) Tracing results of EMSC-EVs (green fluorescence). ( i ) Venn diagram. ( j ) Volcano plot. k . Bubble plot of pathway enrichment analysis. l . miR-665 expression in UMSC-EVs and EMSC-EVs. m . Preliminary screening of miRNAs. n . Target prediction of miR-665 via miRDB database. o . Mechanism of miR-665 targeting STAT3 mRNA. p . The results of the dual-luciferase reporter assay. ( n = 3 per group; ns = not significant, *** P < 0.001, **** P < 0.0001)

Article Snippet: After transfer to polyvinylidene difluoride membranes, rabbit antibodies against phosphorylated STAT3 ( p -STAT3) (1∶2000, CST), mouse antibody against stat3 (1∶2000, CST), mouse antibody against β-actin (1∶1000, Beyotime), and mouse antibody against STAT3 (1∶1000, Beyotime) were used.

Techniques: Isolation, Staining, Fluorescence, Expressing, Luciferase, Reporter Assay

Journal: Journal of Nanobiotechnology

Article Title: ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata

doi: 10.1186/s12951-026-04214-7

Figure Lengend Snippet: ( a ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs. ( b ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs before and after IFN-γ treatment. ( c ) STAT3 expression in IFN-γ-treated HaCaT cells and DPCs. ( d ) WB results showing STAT3 expression in lentivirus-transfected HaCaT cells before and after IFN-γ treatment. ( e ) WB results showing STAT3 expression in lentivirus-transfected DPCs before and after IFN-γ treatment. ( f ) Scratch assay results of HaCaT cells. ( g ) 48 h Transwell assay results of DPCs. ( h ) WB results of rescue experiments in HaCaT cells and DPCs. ( i ) Growth of hair follicles in ex vivo culture on day 5 under different treatment conditions. ( n = 3–6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: After transfer to polyvinylidene difluoride membranes, rabbit antibodies against phosphorylated STAT3 ( p -STAT3) (1∶2000, CST), mouse antibody against stat3 (1∶2000, CST), mouse antibody against β-actin (1∶1000, Beyotime), and mouse antibody against STAT3 (1∶1000, Beyotime) were used.

Techniques: Expressing, Transfection, Wound Healing Assay, Transwell Assay, Ex Vivo